Se Overview PrevMeas
Selenium is an essential nutrient of fundamental importance to human biology. It is an essential constituent of more than two dozen selenoproteins that play critical roles in reproduction, thyroid hormone metabolism, DNA synthesis, and protection from oxidative damage and infection . Thus the determination of selenium at trace and ultra-trace levels has become of increasing importance in life sciences [2–5]. The 82Se/76Se ratio remains relatively constant for ores, but in plants and soil varies from -1.2% to +0.2% . The variations of 82Se/76Se ratio in plant material are believed to be a result of different bacteria residing in the plants. Since plants extract chemical elements directly from the soil during the growing phase, it is reasonable to expect that 82Se/76Se ratio in plants will reflect 82Se/76Se ratio in the soil. Thus the ratio of 82Se/76Se in the plants can be used to fingerprint the geographic region in which they were grown. In the past PAA was used to find a correlation between concentration of some trace elements in coffee and the soil it was grown in.
The concentration of trace elements nutritive importance and toxic effects in biological materials can be determined using different analytical techniques such as inductively coupled plasma mass spectrometry (ICP-MS), atomic absorption spectrometry (AAS), and RNAA [7, 8]. Unfortunately, they require a series of subsequent dissolution and chemical separation and thus have the inherent possibility of losing analytes or altering the contamination level.
PAA has been established for more than fifty years [9, 10], and became more commonly used by the 1980s, when high intensity photon sources became available . Sample preparation for PAA is simple and for most of the samples chemical separation is not necessary. As a result, PAA developed into a relatively common tool for a variety of analytical problems, particularly where high sensitivity is required. Photon activation analysis of selenium has been done in the past using either 75Se or 81,81mSe radioisotopes. 75Se has a relatively long half-life of ~119 days, and requires long irradiation and long cooling time [12, 13]. Both 81Se and 81mSe are rather short-lived (T1/2 < 1 hour) and have also been used in PAA [14, 15]. The goal of this paper is to demonstrate that PAA can be an accurate tool to measure selenium concentration and the 82Se/76Se ratio in soil samples and find its detection limit.
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